1M3

Molecular Biology Laboratory
Return to the Department of Experimental Biology

Specialized equipment includes:

JEM 2100 FE-TEM

The JEM 2100 FE-TEM, a field-emission gun transmission electron microscope, is a state-of-the-art and fully equipped ultra-high resolution analytical TEM that is capable of providing high spatial resolution atomic imaging and microstructure analysis of material samples.

This microscope uses a high-brightness and high-coherence electron beam to carry out ultra-high resolution microchemical analysis using its attached energy dispersive x-ray spectrometer (EDS) and electron energy loss spectrometer (EELS). The 2100 FEG-TEM is also equipped with a scanning image observation device, thus becoming a so-called scanning transmission electron microscope (STEM). This enables researchers to observe and investigate samples in a scanning mode that further expands its applications; for instance, to acquire a material’s chemical composition from a small point or a line profile, or to reveal elemental distribution maps.

This FEG-TEM is also equipped with a Gatan imaging filter that comes with sophisticated software covering all aspects of energy-filtered imaging and energy-loss spectroscopy (EELS). Energy-filtering is a powerful technique that can dramatically improve image contrast and resolution in the TEM as well as quickly obtain highly sensitive elemental maps of materials. By contrast, energy-filtering transmission electron microscopy (EFTEM) is capable of producing electron images from only a narrow range of energies.

Specifications:

  • Accelerating Voltage: 160, 200 kV
  • Electron Gun: ZrO/W(100) Schottky field emission electron source
  • Scanning System: JEOL SIOD digital scanning system with B-F and D-F detectors
  • Resolution:
    o 0.19 nm (point-to-point)
    o 0.10 nm (lattice)
    o 0.2 nm (STEM lattice)
  • Spot Size:
    o 2—5 nm (TEM mode)
    o 0.5—2.4 nm (Analytical mode)
  • Specimen Tilt: +/- 25° (X/Y)
  • Image Recording: CCD and/or film
  • EDS: Oxford (INCA 250) system
  • EELS/Imaging Filter: Gatan (863 GIF Tridiem) system
  • CCD cameras (2): Orius 1000, 2.6k x 4k; Ultrascan 1000, 2k x 2k

Coulter EPICS ALTRA Flow Cytometer and Sort System

Key features:

  • EPICS ALTRA flow cytometer (for 8 colour analysis)
  • Coherent Enterprise II 621 Laser (488 nm and 633 nm)
  • Air Cooled HeNe Laser (633 nm)
  • Sort System (10,000 cells/sec)
  • EXPO2 Cytometry Software

Olympus BX51 Digital Image Analysis Fluorescence Microscope

Key features:

  • BX51 Olympus microscope
  • UPLFL 10x objective
  • UPLFL 20x objective
  • Plan 50x oil objective
  • UPLFL 100x oil objective
  • BX-RFA Fluorescence illuminator
  • U-MWIY2 wide band fluorescence cube (exciter BP 545/580 nm, DM 600, Barrier BA 610 nm)
  • U-MNU2 narrow band fluorescence cube (exciter BP 360/370 nm, DM 500, Barrier BA 420 nm)
  • U-MNB2 narrow band fluorescence cube (exciter 470/490 nm, DM500, Barrier BA 515 nm)
  • DP50 Digital camera (5.8 million pixel equivalent)
  • AnalySIS-Auto software for 3D image construction
  • 733 mHz computer

Leica DM IL inverted phase microscope

Key features:

  • Integrated Hoffman Modulation Contrast (IMC)
  • Optimized Fluorescence with 3 position filter cube slider
  • Field of View: 20 mm
  • 6 Volt/35 Watt transmitted light illumination

1M3

The Dark Queen of the West Rase Cidraen gaaran